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July 1, 2024

Enzyme Conversion

Enzyme Conversion

Biosynthesis of Neurotransmitters from Tyrosine and Tryptophan

Dopamine Biosynthesis from Tyrosine

Tyrosine is an amino acid precursor for several key neurotransmitters, including dopamine. The biosynthesis of dopamine involves the following steps:

  1. Tyrosine Hydroxylation:

Enzyme: Tyrosine hydroxylase (TH)

Reaction: Tyrosine is hydroxylated to L-DOPA (L-3,4-dihydroxyphenylalanine).

Cofactors: Tetrahydrobiopterin (BH4), O2

   Equation: Tyrosine + BH4 + O2 → L-DOPA + BH2 + H2O

Enzyme Conversion

Enzyme Conversion

  1. Decarboxylation of L-DOPA:

Enzyme: Aromatic L-amino acid decarboxylase (AADC), also known as DOPA decarboxylase

Reaction: L-DOPA is decarboxylated to dopamine.

Cofactors: Pyridoxal phosphate (vitamin B6)

Equation: L-DOPA → Dopamine + CO2

Norepinephrine Biosynthesis from Tyrosine

Norepinephrine is synthesized from dopamine through the following steps:

  1. Dopamine Hydroxylation:

   Enzyme: Dopamine β-hydroxylase (DBH)

   Reaction: Dopamine is hydroxylated to norepinephrine.

Cofactors: Ascorbate (vitamin C), O2, Cu2+

Equation: Dopamine + Ascorbate + O2 → Norepinephrine + Dehydroascorbate + H2O

Enzyme Conversion

Serotonin Biosynthesis from Tryptophan

Serotonin is synthesized from the amino acid tryptophan through these steps:

  1. Tryptophan Hydroxylation:

Enzyme: Tryptophan hydroxylase (TPH)

Reaction: Tryptophan is hydroxylated to 5-hydroxytryptophan (5-HTP).

Cofactors: Tetrahydrobiopterin (BH4), O2

Equation: Tryptophan + BH4 + O2 → 5-HTP + BH2 + H2O

  1. Decarboxylation of 5-HTP:

   Enzyme: Aromatic L-amino acid decarboxylase (AADC)

Reaction: 5-HTP is decarboxylated to serotonin (5-hydroxytryptamine or 5-HT).

Cofactors: Pyridoxal phosphate (vitamin B6)

Equation: 5-HTP → Serotonin + CO2

Summary of Biosynthetic Pathways

Dopamine:

  1. Tyrosine → (Tyrosine hydroxylase) → L-DOPA
  2. L-DOPA → (Aromatic L-amino acid decarboxylase) → Dopamine

Norepinephrine:

  1. Dopamine → (Dopamine β-hydroxylase) → Norepinephrine

Serotonin:

  1. Tryptophan → (Tryptophan hydroxylase) → 5-HTP
  2. 5-HTP → (Aromatic L-amino acid decarboxylase) → Serotonin

Each of these steps involves specific enzymes and cofactors essential for the proper conversion of precursor amino acids into their respective neurotransmitters. Understanding these pathways is crucial for insights into various physiological and pathological processes in the nervous system.

Enzyme Conversion

Summary of Biosynthetic Pathways

Dopamine:

  1. Tyrosine → (Tyrosine hydroxylase) → L-DOPA
  2. L-DOPA → (Aromatic L-amino acid decarboxylase) → Dopamine

Norepinephrine:

  1. Dopamine → (Dopamine β-hydroxylase) → Norepinephrine

Serotonin:

  1. Tryptophan → (Tryptophan hydroxylase) → 5-HTP
  2. 5-HTP → (Aromatic L-amino acid decarboxylase) → Serotonin

Each of these steps involves specific enzymes and cofactors essential for the proper conversion of precursor amino acids into their respective neurotransmitters. Understanding these pathways is crucial for insights into various physiological and pathological processes in the nervous system. APA.